Recombinant Murine IL-21 1 ReviewsSubmit a Review Product Details Catalogue Number: 210-21 Description: IL-21 is a pleiotropic cytokine produced by CD4+ T cells in response to antigenic stimulation. Its action generally enhances antigen-specific responses of immune cells. The biological effects of IL-21 include: inducing the differentiation of T cell-stimulated B cells into plasma cells and memory B cells; the stimulation of IgG production in conjunction with IL-4; and the induction of apoptotic effects in naïve B cells and stimulated B cells in the absence of T cell signaling. Additionally, IL-21 promotes the anti-tumor activity of CD8+ T cells and NK cells. IL-21 exerts its effect through binding to a specific type I cytokine receptor, IL-21R, which also contains the γ chain (γc) found in other cytokine receptors, including IL-2, IL-4, IL-7, IL-9 and IL-15. The IL-21/IL-21R interaction triggers a cascade of events, which includes activation of the tyrosine kinases JAK1 and JAK3, followed by activation of the transcription factors STAT1 and STAT3. Recombinant Murine IL-21 is a 15.0 kDa protein consisting of 130 amino acid residues. Source: E.coli Synonyms: Interleukin-21, Za11 AA Sequence: MHKSSPQGPD RLLIRLRHLI DIVEQLKIYE NDLDPELLSA PQDVKGHCEH AAFACFQKAK LKPSNPGNNK TFIIDLVAQL RRRLPARRGG KKQKHIAKCP SCDSYEKRTP KEFLERLKWL LQKMIHQHLS Purity: ≥ 98% by SDS-PAGE gel and HPLC analyses. Biological Activity: Assay #1: Measured by its ability to induce STAT3 signaling in U937 Stat3-Luc reporter cells. Assay #2: Determined by its ability to stimulate the proliferation of human ANBL-6 cells. The expected ED50 is ≤ 1.0 ng/ml, corresponding to a specific activity of ≥ 1 x 106 units/mg. Calculated Molecular Weight: 15 kDa Accession Number: Q9ES17 Gene ID: 60505 crossreactivity: Human, Mouse References PubMed SDS CoA Search Product Line Country Of Origin: USA Not for human use. Research Interest CAR T Cell Apoptosis Cancer Immune System Stem Cells & Differentiation product.subtitle.recentcitations First Author Kaplan, M H Title STAT3-dependent IL-21 production from T helper cells regulates hematopoietic progenitor cell homeostasis. Citation Blood; 117(23) pg6198-201 PubMed Id 21505191 First Author Tsukamoto, H Title IL-6-mediated environmental conditioning of defective Th1 differentiation dampens antitumour immune responses in old age. Citation Nature Communications; 6 pg6702 PubMed Id 25850032 First Author Nojima, T Title In-vitro derived germinal centre B cells differentially generate memory B or plasma cells in vivo. Citation Nature Communications; 2 pg465 PubMed Id 21897376 product.subtitle.productreviews Existing reviews Excellent product Works very nice for mouse B cell culture From: Alexander | Date: 15.07.2020 × Excellent product Works very nice for mouse B cell culture From: Alexander | Date: 15.07.2020 Close