Recombinant Murine Sonic Hedgehog (Shh) 0 BewertungenSubmit a Review Produktdetails Katalognummer: 315-22 Beschreibung: Members of the Hedgehog (Hh) family are highly conserved proteins that are widely represented throughout the animal kingdom. The three known mammalian Hh proteins, Sonic (Shh), Desert (Dhh) and Indian (Ihh), are structurally related, and share a high degree of amino acid sequence identity (e.g. Shh and Ihh are 93% identical). The biologically active form of each Hh molecule is obtained by autocatalytic cleavage of their precursor proteins, and each corresponds to approximately one half of the N-terminal portion of the precursor molecule. Although Hh proteins have unique expression patterns and distinct biological roles within their respective regions of secretion, they use the same signaling pathway and can be substituted for one another in experimental systems. Recombinant Murine Sonic Hedgehog is a 20.0 kDa protein consisting of 176 amino acid residues, including an N-terminal Ile-Val-Ile sequence substituted for the naturally occurring, chemically modified Cys residue. Source: E.coli Synonyms: Hhg-1 AA Sequence: IVIGPGRGFG KRRHPKKLTP LAYKQFIPNV AEKTLGASGR YEGKITRNSE RFKELTPNYN PDIIFKDEEN TGADRLMTQR CKDKLNALAI SVMNQWPGVK LRVTEGWDED GHHSEESLHY EGRAVDITTS DRDRSKYGML ARLAVEAGFD WVYYESKAHI HCSVKAENSV AAKSGG Purity: ≥ 90% by SDS-PAGE gel and HPLC analyses. Biological Activity: Determined by its ability to induce alkaline phosphatase production by C3H/10T1/2 (CCL-226) cells. The expected ED50 for this effect is 0.5-1.0 μg/ml. Calculated Molecular Weight: 20 kDa Accession Number: Q62226 Gene ID: 20423 Endotoxin: Endotoxin level is < 0.1 ng/ug of protein (< 1 EU/ug) Note: 250μg is the largest vial size for this product. Larger sizes supplied as multiple 250μg vials. crossreactivity: Mouse, Rat References PubMed SDS Datenblattsuche Product Line Country Of Origin: USA Not for human use. Research Interest Organoids Apoptosis Bone, Skeletal, Cartilage Immune System Neurobiology Stem Cells & Differentiation Wound Healing product.subtitle.recentcitations Erstautor Zhu, Y Titel Deep learning-based predictive identification of neural stem cell differentiation Literaturstelle Nature Communications; 12(1) pg2614 PubMed ID 33972525 Erstautor Adachi, C Titel Sonic hedgehog enhances calcium oscillations in hippocampal astrocytes. Literaturstelle Journal of Biological Chemistry; 294(44) pg16034-16048 PubMed ID 31506300 Erstautor Faria, A V Titel Smoothened-dependent and -independent pathways in mammalian noncanonical Hedgehog signaling. Literaturstelle The Journal of Biological Chemistry; 294(25) pg9787-9798 PubMed ID 30992365
