Neurotrophine

Recombinant Rat GDNF

Product Details

Catalogue Number: 450-51
Description:

GDNF is a disulfide-linked, homodimeric neurotrophic factor structurally related to Artemin, Neurturin and Persephin.  These proteins belong to the cysteine-knot superfamily of growth factors that assume stable dimeric protein structures.  GDNF signals through a multicomponent receptor system, composed of a RET and one of the four GFRα (α1-α4) receptors.  GDNF specifically promotes dopamine uptake and survival, and morphological differentiation of midbrain neurons.  Using a Parkinson’s disease mouse model, GDNF has been shown to improve conditions such as bradykinesia, rigidity, and postural instability.  The functional rat GDNF ligand is a disulfide-linked homodimer consisting of two 15 kDa polypeptide chains called monomers.  Each monomer contains seven conserved cysteine residues, including Cys-101, which is used for inter-chain disulfide bridging, and others that are involved in the intramolecular ring formation known as the cysteine-knot configuration. The calculated molecular weight of Recombinant Rat GDNF is 30.0 kDa.

Source: E.coli

Synonyms: Glial-Derived Neurotrophic Factor, ATF-1

AA Sequence (monomer): MSPDKQAAAL PRRERNRQAA AASPENSRGK GRRGQRGKNR GCVLTAIHLN VTDLGLGYET KEELIFRYCS GSCEAAETMY DKILKNLSRS RRLTSDKVGQ ACCRPVAFDD DLSFLDDSLV YHILRKHSAK RCGCI

Purity: ≥ 98% by SDS-PAGE gel and HPLC analyses.

Biological Activity: Determined by its ability to stimulate the proliferation of rat C6 cells.

Calculated Molecular Weight: 30 kDa

Accession Number: Q07731

Gene ID: 254535

crossreactivity:
Country Of Origin: USA

Not for human use.

Research Interest

product.subtitle.recentcitations

First Author
Nakagawa, T
Title
A multistate stem cell dynamics maintains homeostasis in mouse spermatogenesis.
Citation
Cell Reports; 37(3) pg109875
PubMed Id
First Author
Zhang, X
Title
AKT3 Is a Pivotal Molecule of Cadherin-22 and GDNF Family Receptor-α1 Signal Pathways Regulating Self-Renewal in Female Germline Stem Cells.
Citation
Stem cells (Dayton, Ohio); 37(8) pg1095-1107
PubMed Id
First Author
Ludtmann, M H
Title
α-synuclein oligomers interact with ATP synthase and open the permeability transition pore in Parkinson's disease.
Citation
Nature Communications; 9(1) pg2293
PubMed Id

product.subtitle.productreviews

Existing reviews
Works good for cell culture
We brought this product for mouse stem cell culture.
From:  Xiaoyu | Date:  28.07.2020