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Recombinant Rat TNF-α

Product Details

Catalogue Number: 400-14
Description:

TNF-α is a pleiotropic pro-inflammatory cytokine secreted by various cells, including adipocytes, activated monocytes, macrophages, B cells, T cells and fibroblasts. It belongs to the TNF family of ligands, and signals through two receptors, TNFR1 and TNFR2. TNF-α is cytotoxic to a wide variety of tumor cells, and is an essential factor in mediating the immune response against bacterial infections. TNF-α also plays a role in the induction of septic shock, autoimmune diseases, rheumatoid arthritis, inflammation, and diabetes. Human and murine TNF-α demonstrate significant cross-species reactivity. TNF-α exists in two forms; a type II transmembrane protein, and a mature soluble protein. The TNF-α transmembrane protein is proteolytically cleaved to yield a soluble, biologically active, 17 kDa TNF-α, which forms a non-covalently linked homotrimer in solution. Recombinant Rat TNF-α is a soluble 157 amino acid protein (17.3 kDa) which corresponds to C-terminal extracellular domain of the full length transmembrane protein.

Source: E.coli

Synonyms: Tumor Necrosis Factor, TNFSF2, Cachectin, Differentiation-inducing factor (DIF), Necrosin, Cytotoxin

AA Sequence: MLRSSSQNSS DKPVAHVVAN HQAEEQLEWL SQRANALLAN GMDLKDNQLV VPADGLYLIY SQVLFKGQGC PDYVLLTHTV SRFAISYQEK VSLLSAIKSP CPKDTPEGAE LKPWYEPMYL GGVFQLEKGD LLSAEVNLPK YLDITESGQV YFGVIAL

Purity: ≥ 98% by SDS-PAGE gel and HPLC analyses.

Biological Activity: The ED50 as determined by the cytolysis of murine L929 cells in the presence of actinomycin D is ≤ 0.05 ng/ml, corresponding to a specific activity of ≥ 2 x 107 units/mg.

Calculated Molecular Weight: 17.3 kDa

Accession Number: P16599

Gene ID: 24835

crossreactivity:
Country Of Origin: USA

Not for human use.

Research Interest

product.subtitle.recentcitations

First Author
Angelov, D N
Title
Tenascin-R is antiadhesive for activated microglia that induce downregulation of the protein after peripheral nerve injury: a new role in neuronal protection.
Citation
The Journal of neuroscience : the official journal of the Society for Neuroscience; 18(16) pg6218-29
PubMed Id
First Author
Han, G
Title
The Combination Treatment of Curcumin and Probucol Protects Chondrocytes from TNF-α Induced Inflammation by Enhancing Autophagy and Reducing Apoptosis via the PI3K-Akt-mTOR Pathway.
Citation
Oxidative Medicine And Cellular Longevity; 2021 pg5558066
PubMed Id
First Author
Xue, X
Title
Protective effect of baicalin against pulmonary arterial hypertension vascular remodeling through regulation of TNF-α signaling pathway.
Citation
Pharmacology Research &Amp; Perspectives; 9(1) pge00703
PubMed Id