Produced in Balb/c mice using highly pure recombinant
Human Adiponectin as the immunizing antigen.
This antibody was purified from cell culture by Protein A affinity
Hi-5 Insect cells derived Recombinant Human Adiponectin (PeproTech catalog #450-24)
a sandwich ELISA (assuming 100μl/well), a concentration of 2.0-4.0 μg/ml of
this antibody will detect at least 500 pg/ml of recombinant Human Adiponectin
when used in conjunction with compatible detection reagents at a concentration
of approximately 0.5-1.0 μg/ml.
detect Human Adiponectin by Western Blot analysis this antibody can be used at
a concentration of 1.0-2.0 μg/ml. When used in conjunction with compatible
secondary reagents the detection limit for recombinant Human Adiponectin is
0.5-1.0 ng/lane, under reducing conditions and 1.0-2.0 ng/lane, under non-reducing
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