Produced in mice using highly pure (>98%) recombinant human BMP-7/OP-1 as the immunizing antigen. This IgG1K antibody was purified from cell culture by Protein G affinity chromatography.
CHO cells derived Recombinant Human BMP-7 (PeproTech catalog #120-03)
In a sandwich ELISA (assuming 100µl/well), when used as a capture antibody at a concentration of 2.0-4.0 µg/ml, this antibody will detect at least 100 pg/ml of recombinant human BMP-7/OP-1 when used with PeproTech’s biotinylated antigen-affinity purified anti-hBMP-7/OP-1 (500-P198Bt) as the detection antibody at a concentration of approximately 0.5-1.0 µg/ml.
This antibody stained
formalin-fixed, paraffin-embedded sections of human breast malignant carcinoma. The recommended concentration is 5.0 µg/mL with an overnight incubation at 4˚C. An HRP-labeled polymer detection system was
used with a DAB chromogen. Heat induced
antigen retrieval with a pH 9.0 buffer is recommended. See photo(s).
Optimal concentrations and conditions may vary.
Tissue samples were provided
by the Cooperative Human Tissue Network, which is funded by the National Cancer
Information and photo are
courtesy of Flagship Biosciences, LLC – Histology Services.
Country of Origin: