Produced in BALB/c mice using highly pure (>98%) recombinant human Eotaxin-2 as the immunizing antigen. This IgG1K antibody was purified from ascites fluid by antigen affinity chromatography.
E.coli derived Recombinant Human Eotaxin-2 (CCL24) (PeproTech catalog #300-33)
In a sandwich ELISA (assuming 100μl/well), a concentration of 2.0-4.0 μg/ml of this antibody will detect at least 100 pg/ml of Recombinant Human Eotaxin-2 when used with PeproTech’s Biotinylated Antigen Affinity Purified anti-Human Eotaxin-2 (500-P103GBt) as the detection antibody at a concentration of approximately 0.050-0.10 μg/ml.
To detect hEotaxin-2 by Western Blot analysis this antibody can be used at a concentration of 0.25-0.50 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hEotaxin-2 is 0.5-1.0 ng/lane, under non-reducing conditions.
This antibody stained formalin-fixed, paraffin-embedded sections of human tonsil. The recommended concentration is 10.0 μg/ml with an overnight incubation at 4˚C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 8.0 EDTA buffer is recommended. Optimal concentrations and conditions may vary. Protocol and staining provided by Dr. Lauren Binge, Laboratory of Prof. Charles Mackay, Monash Univeristy, Australia.
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