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Anti-Human Eotaxin-3

Catalog Number:
500-M32
Source:
Monoclonal Mouse
Preparation:
Produced in BALB/c ICRR F1 mice using highly pure (>98%) recombinant human Eotaxin-3 as the immunizing antigen. This IgG2aK antibody was purified from ascites fluid by Protein A affinity chromatography.
Immunogen:
E.coli derived Recombinant Human Eotaxin-3 (CCL26) (PeproTech catalog #300-48)
Application:
ELISA
Sandwich
In a sandwich ELISA (assuming 100µl/well), a concentration of 1.0-2.0 µg/ml of this antibody will detect at least 1000 pg/ml of recombinant human Eotaxin-3 when used with PeproTech's biotinylated antigen affinity purified anti-human Eotaxin-3 (500-P156GBt) as the detection antibody at a concentration of approximately 0.25-0.50 µg/ml.
Western Blot
To detect hEotaxin-3 by Western Blot analysis this antibody can be used at a concentration of 0.25-0.50 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hEotaxin-3 is 0.25-0.5 ng/lane, under reducing or non-reducing conditions.
Neutralization
Research Interests:
Country of Origin:
USA
Research Use Only. Not for use in diagnostic or therapeutic procedures.
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  Size Price Qty
A 500µg 150.00 USD
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