Produced in BALB/c mice using highly pure recombinant Human FGF-5 as the immunizing antigen. This IgG1K antibody was purified from cell culture by Protein A affinity chromatography.
E.coli derived Recombinant Human FGF-5 (PeproTech catalog #100-34)
In a sandwich ELISA (assuming 100µl/well), a concentration of 4.0-8.0 μg/ml of this antibody will detect at least 100 pg/ml of recombinant Human FGF-5 when used in conjunction with compatible secondary reagents.
yield one-half maximal inhibition [ND50]
of the biological activity of hFGF-5 (27.6 μg/ml), a concentration of 100 μg/ml
of this antibody is required.
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