Produced in BALB/c x ICR F1 mice using highly pure recombinant human FGF-basic as the immunizing antigen. This IgG1K antibody was purified from ascites fluid by Protein A affinity chromatography.
E.coli derived Recombinant Human FGF-basic (154 a.a.) (PeproTech catalog #100-18B)
In a sandwich ELISA (assuming 100µl/well), a concentration of 2.0-4.0 µg/ml of this antibody will detect at least 1000 pg/ml of recombinant human FGF-basic when used with PeproTech's biotinylated antigen affinity purified anti-human FGF-basic (500-P18Bt) as the detection antibody at a concentration of approximately 0.125-0.25 µg/ml.
To detect Human FGF-basic by Western Blot analysis this antibody can be used at a concentration of 0.25-0.50 µg/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human FGF-basic is 1.0-2.0 ng/lane, under non-reducing conditions.
This antibody stained formalin-fixed, paraffin-embedded sections of human breast invasive ductal carcinoma. The recommended concentrations are 0.25-0.50 μg/ml overnight at 4˚C. An HRP-labeled polymer detection system was used with DAB chromogen. Heat induced antigen retrieval was performed with a pH 6.0 Sodium Citrate buffer. Optimal concentrations and conditions may vary. Tissue samples were provided by the Cooperative Human Tissue Network which is funded by the National Cancer Institute.
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