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Anti-Human G-CSF

Catalog Number:
500-M37
Source:
Monoclonal Mouse
Preparation:

Produced in Balb/c mice using highly pure Recombinant Human G-CSF as the immunizing antigen.  This antibody was purified from cell culture by Protein A affinity chromatography.

Immunogen:
E.coli derived Recombinant Human G-CSF (PeproTech catalog #AF-300-23)
Application:
ELISA
Sandwich
In a sandwich ELISA (assuming 100μl/well), a concentration of 2.0-4.0 μg/ml of this antibody will detect at least 100 pg/ml of Recombinant Human G-CSF when used with PeproTech’s Biotinylated Antigen Affinity Purified anti-Human G-CSF (500-P43Bt) as the detection antibody, at a concentration of approximately 0.5-1.0 μg/ml.
Western Blot
To detect Human G-CSF by Western Blot analysis, this antibody can be used at a concentration of 0.20-0.40 μg/ml. When used in conjunction with compatible secondary reagents the detection limit for Recombinant Human G-CSF is 1.5-2.0 ng/lane under non-reducing conditions, and 7.5-10.0 ng/lane under reducing conditions.
Neutralization
Research Interests:
Country of Origin:
USA
Research Use Only. Not for use in diagnostic or therapeutic procedures.
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  Size Price Qty
A 500µg 150.00 USD
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