Produced in BALB/c mice using highly pure (>98%) recombinant human IL-1β as the immunizing antigen. This IgG1K antibody was purified from cell culture by Protein G affinity chromatography.
E.coli derived Recombinant Human IL-1β (PeproTech catalog #200-01B)
In a sandwich ELISA (assuming 100μl/well), a concentration of 0.25-0.50 μg/ml of this antibody will detect at least 100 pg/ml of recombinant Human IL-1β when used with PeproTech’s Biotinylated Antigen Affinity Purified Anti-Human IL-1β (500-P21BBt) as the detection antibody at a concentration of approximately 0.25-0.50 μg/ml.
detect Human IL-1β by Western Blot analysis this antibody can be used at a
concentration of 0.25-0.50 μg/ml. When used in conjunction with compatible
secondary reagents, the detection limit for recombinant Human IL-1β is 2.0-4.0
ng/lane, under reducing conditions and 4.0-8.0 ng/lane, under non-reducing
To yield one-half maximal inhibition [ND50] of the biological activity of Human IL-1β (1.00 pg/ml), a concentration of <0.01 μg/ml of this antibody is required.
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