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Anti-Human IL-1β

Catalog Number:
500-M01B
Source:
Monoclonal Mouse
Preparation:
Produced in BALB/c mice using highly pure (>98%) recombinant human IL-1β as the immunizing antigen. This IgG1K antibody was purified from cell culture by Protein G affinity chromatography.
Immunogen:
E.coli derived Recombinant Human IL-1β (PeproTech catalog #200-01B)
Application:
ELISA
Sandwich
In a sandwich ELISA (assuming 100μl/well), a concentration of 0.25-0.50 μg/ml of this antibody will detect at least 100 pg/ml of recombinant Human IL-1β when used with PeproTech’s Biotinylated Antigen Affinity Purified Anti-Human IL-1β (500-P21BBt) as the detection antibody at a concentration of approximately 0.25-0.50 μg/ml.
Western Blot
To detect Human IL-1β by Western Blot analysis this antibody can be used at a concentration of 0.25-0.50 μg/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human IL-1β is 2.0-4.0 ng/lane, under reducing conditions and 4.0-8.0 ng/lane, under non-reducing conditions.
Neutralization
Research Interests:
Country of Origin:
USA
Research Use Only. Not for use in diagnostic or therapeutic procedures.
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  Size Price Qty
A 500µg 150.00 USD
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