Produced in mice using highly pure recombinant Human
IL-3 as the immunizing antigen. This
antibody was purified from tissue culture supernatant by Protein G affinity
E.coli derived Recombinant Human IL-3 (PeproTech catalog #200-03)
a sandwich ELISA (assuming 100μl/well), a concentration of 4.0-8.0 μg/ml of
this antibody will detect at least 500 pg/ml of recombinant Human IL-3 when
used with PeproTech’s Biotinylated Antigen Affinity Purified Anti-Human IL-3 (500-P23Bt)
as the detection antibody at a concentration of approximately 0.50-1.0 μg/ml.
detect Human IL-3 by Western Blot analysis this antibody can be used at a concentration
of 0.25-0.50 μg/ml. When used in conjunction with compatible secondary reagents,
the detection limit for recombinant Human IL-3 is 0.50-1.0 ng/lane, under
reducing conditions and 2.0-4.0 ng/lane, under non-reducing conditions.
yield one-half maximal inhibition [ND50]
of the biological activity of Human IL-3 (1.0 ng/ml), a concentration of 0.007-0.010
μg/ml of this antibody is required.
Country of Origin: