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Anti-Human IL-4

Catalog Number:
500-M04
Source:
Monoclonal Mouse
Preparation:
Produced in BALB/c x ICR F1 mice using highly pure (>98%) recombinant human IL-4 as the immunizing antigen. This IgG1K antibody was purified from ascites fluid by Protein A affinity chromatography.
Immunogen:
E.coli derived Recombinant Human IL-4 (PeproTech catalog #200-04)
Application:
ELISA
Sandwich
In a sandwich ELISA (assuming 100µl/well), a concentration of 0.5-1.0 µg/ml of this antibody will detect at least 2000 pg/ml of recombinant human IL-4 when used with PeproTech's biotinylated antigen affinity purified anti-human IL-4 (500-P24Bt) as the detection antibody at a concentration of approximately 2.0-4.0 µg/ml.
Western Blot
To detect hIL-4 by Western Blot analysis this antibody can be used at a concentration of 0.20-0.50 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-4 is 0.25-1.0 ng/lane, under reducing or non-reducing conditions.
Cross Reactivity:
Mouse
Research Interests:
Country of Origin:
USA
Research Use Only. Not for use in diagnostic or therapeutic procedures.
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  Size Price Qty
A 500µg 150.00 USD
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