Produced in BALB/c mice using highly pure (>98%) recombinant rat IL-2 as the immunizing antigen. This IgG1K antibody was purified from cell culture by Protein A affinity chromatography.
E.coli derived Recombinant Rat IL-2 (PeproTech catalog #400-02)
In a sandwich ELISA (assuming 100µl/well), a concentration of 14.0-16.0 µg/ml of this antibody will detect at least 1000 pg/ml of recombinant rat IL-2 when used with PeproTech's biotinylated antigen affinity purified anti-rat IL-2 (500-P274Bt) as the detection antibody at a concentration of at least 2.0-4.0 µg/ml.
To detect rat IL-2 by Western Blot analysis this antibody can be used at a concentration of 0.25-0.50 µg/ml. When used in conjunction with compatible secondary reagents the detection limit for recombinant rat IL-2 is 2.0-4.0 ng/lane, under reducing or non-reducing conditions.
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