Glial-Derived Neurotrophic Factor, ATF-1
GDNF is a disulfide-linked,
homodimeric neurotrophic factor structurally related to Artemin, Neurturin and
Persephin. These proteins belong to the
cysteine-knot superfamily of growth factors that assume stable dimeric protein
structures. GDNF signals through a
multicomponent receptor system, composed of a RET and one of the four GFRα
(α1-α4) receptors. GDNF specifically
promotes dopamine uptake and survival, and morphological differentiation of
midbrain neurons. Using a Parkinson’s
disease mouse model, GDNF has been shown to improve conditions such as
bradykinesia, rigidity, and postural instability. The functional human GDNF ligand is a
disulfide-linked homodimer consisting of two 15 kDa polypeptide chains called
monomers. Each monomer contains seven
conserved cysteine residues, including Cys-101, which is used for inter-chain
disulfide bridging, and others that are involved in the intramolecular ring formation
known as the cysteine knot configuration. The calculated molecular weight of Recombinant Human GDNF is 30.4 kDa.
MSPDKQMAVL PRRERNRQAA AANPENSRGK GRRGQRGKNR GCVLTAIHLN VTDLGLGYET KEELIFRYCS GSCDAAETTY DKILKNLSRN RRLVSDKVGQ ACCRPIAFDD DLSFLDDNLV YHILRKHSAK RCGCI
Greater than 98% by SDS-PAGE gel and HPLC analyses.
The ED50 was determined by the proliferation of rat C6 cells is ≤ 0.1 μg/ml, corresponding to a specific activity of ≥ 1 x 104 units/mg.
Frog, Monkey, Mouse, Rat, Human, N/A, Human + Virus, Human + Mouse
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