Antigen Affinity Purified Polyclonal Antibodies

Anti-Human/Murine/Rat BDNF

Product Details

Catalogue Number: 500-P84

Source: Polyclonal Rabbit

Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human/Murine/Rat BDNF. Anti-Human/Murine/Rat BDNF-specific antibody was purified by affinity chromatography employing immobilized Human/Murine/Rat BDNF matrix.

Immunogen: E.coli derived Recombinant Human/Murine/Rat BDNF (PeproTech catalog# 450-02)

Sandwich ELISA: To detect hBDNF by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech’s Biotinylated Anti-Human BDNF (500-P84Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hBDNF.

Anti-Human/Murine/Rat BDNF Sandwich ELISA

Western Blot: To detect hBDNF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hBDNF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Anti-Human/Murine/Rat BDNF Western Blot Reduced Anti-Human/Murine/Rat BDNF Western Blot Unreduced

Note:

Additional applications tested on a lot-to-lot basis. Please contact Technical Support for more information.

crossreactivity:
Country Of Origin: USA

Not for human use.

Research Interest

product.subtitle.recentcitations

First Author
Peng, T
Title
Keratinocytes produce IL-17c to protect peripheral nervous systems during human HSV-2 reactivation.
Citation
The Journal of Experimental Medicine; 214(8) pg2315-2329
PubMed Id
First Author
Boukhatem, I
Title
The brain-derived neurotrophic factor prompts platelet aggregation and secretion.
Citation
Blood Advances; 5(18) pg3568-3580
PubMed Id
First Author
Wirth, M J
Title
Transcellular induction of neuropeptide Y expression by NT4 and BDNF.
Citation
Proceedings of the National Academy of Sciences of the United States of America; 102(8) pg3064-9
PubMed Id

product.subtitle.productreviews

Existing reviews
BDNF for organoids
Works well.
From:  Dawn | Date:  7/14/2020