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Antigen Affinity Purified Polyclonal Antibodies

Anti-Human/Murine/Rat BDNF

Product Details

Catalog Number: 500-P84

Source: Polyclonal Rabbit

Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human/Murine/Rat BDNF. Anti-Human/Murine/Rat BDNF-specific antibody was purified by affinity chromatography employing immobilized Human/Murine/Rat BDNF matrix.

Immunogen: E.coli derived Recombinant Human/Murine/Rat BDNF (PeproTech catalog# 450-02)

Sandwich ELISA: To detect hBDNF by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech’s Biotinylated Anti-Human BDNF (500-P84Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hBDNF.

Anti-Human/Murine/Rat BDNF Sandwich ELISA

Western Blot: To detect hBDNF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hBDNF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Anti-Human/Murine/Rat BDNF Western Blot Reduced Anti-Human/Murine/Rat BDNF Western Blot Unreduced


Additional applications tested on a lot-to-lot basis. Please contact Technical Support for more information.

Cross Reactivity Cited in References: Research Interest

Country Of Origin: USA

Not for human use.

Product Reviews

Existing reviews
BDNF for organoids
Works well.
From:  Dawn | Date:  7/14/2020