Antigen Affinity Purified Polyclonal Antibodies

Anti-Human Eotaxin (CCL11) (Polyclonal Rabbit)

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Product Details

Catalogue Number: 500-P41
Description:
Anti-Human Eotaxin (CCL11) (Polyclonal Rabbit)
 

Source: Polyclonal Rabbit

Preparation: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hEotaxin. Anti-Human Eotaxin specific antibody was purified by affinity chromatography employing immobilized hEotaxin matrix.

Immunogen: E.coli derived Recombinant Human Eotaxin (CCL11) (PeproTech catalog# 300-21)

Sandwich ELISA: To detect hEotaxin by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech’s Biotinylated Anti-Human Eotaxin (500-P41Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hEotaxin.

Anti-Human Eotaxin (CCL11) Sandwich ELISA

Western Blot: To detect hEotaxin by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hEotaxin is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Anti-Human Eotaxin (CCL11) Western Blot Reduced Anti-Human Eotaxin (CCL11) Western Blot Unreduced

Note:

Additional applications tested on a lot-to-lot basis. Please contact Technical Support for more information.

crossreactivity:
Country Of Origin: USA

Not for human use.

Research Interest

product.subtitle.recentcitations

First Author
Thielen, C
Title
TARC and IL-5 expression correlates with tissue eosinophilia in peripheral T-cell lymphomas.
Citation
Leukemia Research; 32(9) pg1431-8
PubMed Id
First Author
Blumenthal, R D
Title
Degranulating eosinophils in human endometriosis.
Citation
The American Journal of Pathology; 156(5) pg1581-8
PubMed Id
First Author
Teruya-Feldstein, J
Title
Differential chemokine expression in tissues involved by Hodgkin's disease: direct correlation of eotaxin expression and tissue eosinophilia.
Citation
Blood; 93(8) pg2463-70
PubMed Id