Anti-Human GDNF 0 ReviewsSubmit a Review Product Details Catalog Number: 500-P81 Description: Source: Polyclonal Rabbit Preparation: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hGDNF. Anti-Human GDNF specific antibody was purified by affinity chromatography employing immobilized hGDNF matrix. Immunogen: E.coli derived Recombinant Human GDNF (PeproTech catalog# 450-10) Sandwich ELISA: To detect hGDNF by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech’s Biotinylated Anti-Human GDNF (500-P81Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hGDNF. Western Blot: To detect hGDNF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hGDNF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. Note: Additional applications tested on a lot-to-lot basis. Please contact Technical Support for more information. Cross Reactivity Cited in References: Human, Rat References SDS CoA Search Product Line Country Of Origin: USA Not for human use. Research Interest Angiogenesis/Cardiovascular Neurobiology Stem Cells & Differentiation TGF-β Superfamily Recent Citations First Author Watanabe, Y Title Use of a Human Artificial Chromosome for Delivering Trophic Factors in a Rodent Model of Amyotrophic Lateral Sclerosis. Citation Molecular therapy. Nucleic acids; 4 pge253 PubMed ID 26440597 First Author Li, A Title Apomorphine-induced activation of dopamine receptors modulates FGF-2 expression in astrocytic cultures and promotes survival of dopaminergic neurons. Citation FASEB journal : official publication of the Federation of American Societies for Experimental Biology; 20(8) pg1263-5 PubMed ID 16636101