Antigen Affinity Purified Polyclonal Antibodies

Anti-Human IFN-γ

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Product Details

Catalogue Number: 500-P32

Source: Polyclonal Rabbit

Preparation: Produced from sera of rabbits pre-immunized with highly pure recombinant Human IFN-γ. Anti-Human IFN-γ specific antibody was purified by affinity chromatography employing immobilized Human IFN-γ matrix.

Immunogen: E.coli derived Recombinant Human IFN-γ (PeproTech catalog# 300-02)

Sandwich ELISA: To detect Human IFN-γ by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech’s Biotinylated Anti-Human IFN-γ (500-P32Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human IFN-γ.

Anti-Human IFN-γ Sandwich ELISA

Western Blot: To detect Human IFN-γ by Western Blot analysis this antibody can be used at a concentration of 0.1- 0.2 µg/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human IFN-γ is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Anti-Human IFN-γ Western Blot Reduced Anti-Human IFN-γ Western Blot Unreduced

Note:

Additional applications tested on a lot-to-lot basis. Please contact Technical Support for more information.

crossreactivity:
Country Of Origin: USA

Not for human use.

Research Interest

product.subtitle.recentcitations

First Author
Murakami, K
Title
Production of biologically active recombinant bovine interferon-gamma by two different baculovirus gene expression systems using insect cells and silkworm larvae.
Citation
Cytokine; 13(1) pg18-24
PubMed Id
First Author
Sanderson, N S
Title
Cytotoxic immunological synapses do not restrict the action of interferon-γ to antigenic target cells.
Citation
Proceedings of the National Academy of Sciences of the United States of America; 109(20) pg7835-40
PubMed Id
First Author
O'Neil, D
Title
IFN-gamma down-regulates MHC expression and antigen processing in a human B cell line.
Citation
Journal of immunology (Baltimore, Md. : 1950); 162(2) pg791-8
PubMed Id