Antigen Affinity Purified Polyclonal Antibodies

Anti-Human IL-15

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Product Details

Catalogue Number: 500-P15

Source: Polyclonal Rabbit

Preparation: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hIL-15. Anti-Human IL-15 specific antibody was purified by affinity chromatography employing immobilized hIL-15 matrix.

Immunogen: E.coli derived Recombinant Human IL-15 (PeproTech catalog# 200-15)

Sandwich ELISA: To detect hIL-15 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech’s Biotinylated Anti-Human IL-15 (500-P15Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-15.

Anti-Human IL-15 Sandwich ELISA

Western Blot: To detect hIL-15 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-15 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Anti-Human IL-15 Western Blot Reduced Anti-Human IL-15 Western Blot Unreduced

Note:

Additional applications tested on a lot-to-lot basis. Please contact Technical Support for more information.

crossreactivity:
Country Of Origin: USA

Not for human use.

Research Interest

product.subtitle.recentcitations

First Author
Kim, Y S
Title
Targeting the IL-15 receptor with an antagonist IL-15 mutant/Fc gamma2a protein blocks delayed-type hypersensitivity.
Citation
Journal of immunology (Baltimore, Md. : 1950); 160(12) pg5742-8
PubMed Id
First Author
Qin, J Z
Title
Interleukin-7 and interleukin-15 regulate the expression of the bcl-2 and c-myb genes in cutaneous T-cell lymphoma cells.
Citation
Blood; 98(9) pg2778-83
PubMed Id
First Author
Döbbeling, U
Title
Interleukin-15 is an autocrine/paracrine viability factor for cutaneous T-cell lymphoma cells.
Citation
Blood; 92(1) pg252-8
PubMed Id