Anti-Human IL-21 1 ReviewsSubmit a Review Product Details Catalog Number: 500-P191 Description: Source: Polyclonal Rabbit Preparation: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hIL-21. Anti-Human IL-21 specific antibody was purified by affinity chromatography employing immobilized hIL-21 matrix. Immunogen: E.coli derived Recombinant Human IL-21 (PeproTech catalog# 200-21) Sandwich ELISA: To detect hIL-21 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech’s Biotinylated Anti-Human IL-21 (500-P191Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-21. Western Blot: To detect hIL-21 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-21 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. Note: Additional applications tested on a lot-to-lot basis. Please contact Technical Support for more information. Cross Reactivity Cited in References: Human References SDS CoA Search Product Line Country Of Origin: USA Not for human use. Research Interest Apoptosis Cancer Immune System Stem Cells & Differentiation Recent Citations First Author Xing, X Title IFN-γ+ IL-17+ Th17 cells regulate fibrosis through secreting IL-21 in systemic scleroderma. Citation Journal of Cellular and Molecular Medicine; 24(23) pg13600-13608 PubMed ID 33157566 First Author Nagy, N Title Soluble factors produced by activated CD4+ T cells modulate EBV latency. Citation Proceedings of the National Academy of Sciences of the United States of America; 109(5) pg1512-7 PubMed ID 22307606 First Author Ma, C S Title Early commitment of naïve human CD4(+) T cells to the T follicular helper (T(FH)) cell lineage is induced by IL-12. Citation Immunology and Cell Biology; 87(8) pg590-600 PubMed ID 19721453 Product Reviews Existing reviews great product cleanest form of IL-21 we have used From: Thomas | Date: 8/26/2020 × great product cleanest form of IL-21 we have used From: Thomas | Date: 8/26/2020 Close
