Antigen Affinity Purified Polyclonal Antibodies

Anti-Human IL-22

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Product Details

Catalogue Number: 500-P211

Source: Polyclonal Rabbit

Preparation: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hIL-22. Anti-Human IL-22 specific antibody was purified by affinity chromatography employing immobilized hIL-22 matrix.

Immunogen: E.coli derived Recombinant Human IL-22 (PeproTech catalog# 200-22)

Sandwich ELISA: To detect hIL-22 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech’s Biotinylated Anti-Human IL-22 (500-P211Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-22.

Anti-Human IL-22 Sandwich ELISA

Western Blot: To detect hIL-22 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-22 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Anti-Human IL-22 Western Blot Reduced Anti-Human IL-22 Western Blot Unreduced

Note:

Additional applications tested on a lot-to-lot basis. Please contact Technical Support for more information.

crossreactivity:
Country Of Origin: USA

Not for human use.

Research Interest

product.subtitle.recentcitations

First Author
Bouhaddou, M
Title
The Global Phosphorylation Landscape of SARS-CoV-2 Infection.
Citation
Cell; 182(3) pg685-712.e19
PubMed Id
First Author
Kan, A A
Title
Protein expression profiling of inflammatory mediators in human temporal lobe epilepsy reveals co-activation of multiple chemokines and cytokines.
Citation
Journal of Neuroinflammation; 9 pg207
PubMed Id
First Author
Hedl, M
Title
JAK2 Disease-Risk Variants Are Gain of Function and JAK Signaling Threshold Determines Innate Receptor-Induced Proinflammatory Cytokine Secretion in Macrophages.
Citation
Journal of immunology (Baltimore, Md. : 1950); 197(9) pg3695-3704
PubMed Id