Produced from sera of rabbits immunized with highly pure recombinant Human LIF. Anti-Human LIF specific antibody was purified by affinity chromatography employing an immobilized Human LIF matrix.
E.coli derived Recombinant Human LIF (PeproTech catalog# 300-05)
To detect Human LIF by sandwich ELISA (using 100μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech’s Biotinylated Anti-Human LIF (500-P39Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human LIF.
To detect Human LIF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/ml. When used in conjunction with compatible secondary reagents the detection limit for recombinant Human LIF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Additional applications tested on a lot-to-lot basis. Please contact Technical Support for more information.