PeproTech is proud to be able to continue to support you in the midst of unprecedented challenges pertaining to the COVID-19 virus. For more information, click here.

Antigen Affinity Purified Polyclonal Antibodies

Anti-Human M-CSF

Product Details

Catalog Number: 500-P44

Source: Polyclonal Rabbit

Preparation: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hM-CSF. Anti-Human M-CSF specific antibody was purified by affinity chromatography employing immobilized hM-CSF matrix.

Immunogen: E.coli derived Recombinant Human M-CSF (PeproTech catalog# 300-25)

Sandwich ELISA: To detect hM-CSF by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech’s Biotinylated Anti-Human M-CSF (500-P44Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hM-CSF.

Anti-Human M-CSF Sandwich ELISA

Western Blot: To detect hM-CSF by Western Blot analysis this antibody can be used at a concentration of 0.1- 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hM-CSF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Anti-Human M-CSF Western Blot Reduced Anti-Human M-CSF Western Blot Unreduced


Additional applications tested on a lot-to-lot basis. Please contact Technical Support for more information.

Cross Reactivity Cited in References: Research Interest

Country Of Origin: USA

Not for human use.

Product Reviews

Existing reviews
Tested and worked great
My lab and I have used this product for years now for development of human macrophages. This always works and gives us the desired results.
From:  Pooja | Date:  8/26/2020