Antigen Affinity Purified Polyclonal Antibodies

Anti-Human M-CSF

Product Details

Catalogue Number: 500-P44
Description:
Anti-Human M-CSF
 

Source: Polyclonal Rabbit

Preparation: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hM-CSF. Anti-Human M-CSF specific antibody was purified by affinity chromatography employing immobilized hM-CSF matrix.

Immunogen: E.coli derived Recombinant Human M-CSF (PeproTech catalog# 300-25)

Sandwich ELISA: To detect hM-CSF by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech’s Biotinylated Anti-Human M-CSF (500-P44Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hM-CSF.

Anti-Human M-CSF Sandwich ELISA

Western Blot: To detect hM-CSF by Western Blot analysis this antibody can be used at a concentration of 0.1- 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hM-CSF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Anti-Human M-CSF Western Blot Reduced Anti-Human M-CSF Western Blot Unreduced

Note:

Additional applications tested on a lot-to-lot basis. Please contact Technical Support for more information.

crossreactivity:
Country Of Origin: USA

Not for human use.

Research Interest

product.subtitle.recentcitations

First Author
Sánchez-Martín, L
Title
The chemokine CXCL12 regulates monocyte-macrophage differentiation and RUNX3 expression.
Citation
Blood; 117(1) pg88-97
PubMed Id
First Author
Tan, B L
Title
Genetic evidence for convergence of c-Kit- and alpha4 integrin-mediated signals on class IA PI-3kinase and the Rac pathway in regulating integrin-directed migration in mast cells.
Citation
Blood; 101(12) pg4725-32
PubMed Id

product.subtitle.productreviews

Existing reviews
Tested and worked great
My lab and I have used this product for years now for development of human macrophages. This always works and gives us the desired results.
From:  Pooja | Date:  8/26/2020