Anti-Human M-CSF 1 ReviewsSubmit a Review Product Details Catalog Number: 500-P44 Description: Source: Polyclonal Rabbit Preparation: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hM-CSF. Anti-Human M-CSF specific antibody was purified by affinity chromatography employing immobilized hM-CSF matrix. Immunogen: E.coli derived Recombinant Human M-CSF (PeproTech catalog# 300-25) Sandwich ELISA: To detect hM-CSF by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech’s Biotinylated Anti-Human M-CSF (500-P44Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hM-CSF. Western Blot: To detect hM-CSF by Western Blot analysis this antibody can be used at a concentration of 0.1- 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hM-CSF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. Note: Additional applications tested on a lot-to-lot basis. Please contact Technical Support for more information. Cross Reactivity Cited in References: Human References SDS CoA Search Product Line Country Of Origin: USA Not for human use. Research Interest Cancer Proliferation Stem Cells & Differentiation Recent Citations First Author Sánchez-Martín, L Title The chemokine CXCL12 regulates monocyte-macrophage differentiation and RUNX3 expression. Citation Blood; 117(1) pg88-97 PubMed ID 20930067 First Author Tan, B L Title Genetic evidence for convergence of c-Kit- and alpha4 integrin-mediated signals on class IA PI-3kinase and the Rac pathway in regulating integrin-directed migration in mast cells. Citation Blood; 101(12) pg4725-32 PubMed ID 12560232 Product Reviews Existing reviews Tested and worked great My lab and I have used this product for years now for development of human macrophages. This always works and gives us the desired results. From: Pooja | Date: 8/26/2020 × Tested and worked great My lab and I have used this product for years now for development of human macrophages. This always works and gives us the desired results. From: Pooja | Date: 8/26/2020 Close
