Antigen Affinity Purified Polyclonal Antibodies

Anti-Human MCP-4 (CCL13) (Polyclonal Rabbit)

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Product Details

Catalogue Number: 500-P04

Source: Polyclonal Rabbit

Preparation: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hMCP-4. Anti-Human MCP-4 specific antibody was purified by affinity chromatography employing immobilized hMCP-4 matrix.

Immunogen: E.coli derived Recombinant Human MCP-4 (CCL13) (PeproTech catalog# 300-24)

Sandwich ELISA: To detect Human MCP-4 by sandwich ELISA (using 100μl/well) a concentration of 0.5-2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech’s Biotinylated Anti-Human MCP-4 (CCL13) (500-P04BT) as a detection antibody, allows the detection of at least 2000- 4000 pg/ml of recombinant Human MCP-4.

Anti-Human MCP-4 (CCL13) (Polyclonal Rabbit) Sandwich ELISA Anti-Human MCP-4 (CCL13) (Polyclonal Rabbit) Sandwich ELISA

Western Blot: To detect Human MCP-4 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/ml. When used in conjunction with compatible secondary reagents the detection limit for Recombinant Human MCP-4 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Anti-Human MCP-4 (CCL13) Western Blot Reduced Anti-Human MCP-4 (CCL13) Western Blot Unreduced Anti-Human MCP-4 (CCL13) Western Blot Reduced Anti-Human MCP-4 (CCL13) Western Blot Unreduced

Note:

Additional applications tested on a lot-to-lot basis. Please contact Technical Support for more information.

crossreactivity:
Country Of Origin: USA

Not for human use.

Research Interest

product.subtitle.recentcitations

First Author
Mateo, T
Title
Angiotensin II-induced mononuclear leukocyte interactions with arteriolar and venular endothelium are mediated by the release of different CC chemokines.
Citation
Journal of immunology (Baltimore, Md. : 1950); 176(9) pg5577-86
PubMed Id