Antigen Affinity Purified Polyclonal Antibodies

Anti-Human Midkine

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Product Details

Catalogue Number: 500-P171

Source: Polyclonal Rabbit

Preparation: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hMidkine. Anti-Human Midkine specific antibody was purified by affinity chromatography employing immobilized hMidkine matrix.

Immunogen: E.coli derived Recombinant Human Midkine (PeproTech catalog# 450-16)

Sandwich ELISA: To detect hMidkine by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech’s Biotinylated Anti-Human Midkine (500-P171Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hMidkine.

Anti-Human Midkine Sandwich ELISA

Western Blot: To detect hMidkine by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMidkine is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Anti-Human Midkine Western Blot Reduced Anti-Human Midkine Western Blot Unreduced


Additional applications tested on a lot-to-lot basis. Please contact Technical Support for more information.

Country Of Origin: USA

Not for human use.

Research Interest


First Author
Kuo, A H
Recruitment of insulin receptor substrate-1 and activation of NF-kappaB essential for midkine growth signaling through anaplastic lymphoma kinase.
Oncogene; 26(6) pg859-69
PubMed Id
First Author
Zhou, Z
NGPF2 triggers synaptic scaling up through ALK-LIMK-cofilin-mediated mechanisms.
Cell Reports; 36(7) pg109515
PubMed Id
First Author
You, Z
Midkine is a NF-kappaB-inducible gene that supports prostate cancer cell survival.
BMC Medical Genomics; 1 pg6
PubMed Id