Antigen Affinity Purified Polyclonal Antibodies

Source: Polyclonal Rabbit

Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human NT-4. Anti-Human NT-4-specific antibody was purified by affinity chromatography employing an immobilized Human NT-4 matrix.

Immunogen: E.coli-derived, 28.1kDa Recombinant Human NT-4 (PeproTech Catalog# 450-04)

Sandwich ELISA: To detect Human NT-4 by sandwich ELISA (using 100ul/well) a concentration of 0.5-2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech’s Biotinylated Anti-Human NT-4 (500-P83BT) as a detection antibody, allows the detection of at least 2000-4000 pg/ml of Recombinant Human NT-4.

Western Blot: To detect Human NT-4 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2ug/ml. When used in conjunction with compatible secondary reagents the detection limit for Recombinant Human NT-4 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Note:

Additional applications tested on a lot-to-lot basis. Please contact Technical Support for more information.