Antigen Affinity Purified Polyclonal Antibodies

Anti-Human sRANK Ligand (Polyclonal Rabbit)

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Product Details

Catalogue Number: 500-P133

Source: Polyclonal Rabbit

Preparation: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hsRANKL. Anti-Human sRANKL specific antibody was purified by affinity chromatography employing immobilized hsRANKL matrix.

Immunogen: E.coli derived Recombinant Human sRANK Ligand (PeproTech catalog# 310-01)

Sandwich ELISA: To detect hsRANKL by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech’s Biotinylated Anti-Human sRANKL (500-P133Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hsRANKL.

Anti-Human sRANK Ligand Sandwich ELISA

Western Blot: To detect hsRANKL by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hsRANKL is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Anti-Human sRANK Ligand Western Blot Reduced Anti-Human sRANK Ligand Western Blot Unreduced

Note:

Additional applications tested on a lot-to-lot basis. Please contact Technical Support for more information.

crossreactivity:
Country Of Origin: USA

Not for human use.

Research Interest

product.subtitle.recentcitations

First Author
Martínez-Calatrava, M J
Title
RANKL synthesized by articular chondrocytes contributes to juxta-articular bone loss in chronic arthritis.
Citation
Arthritis research & therapy; 14(3) pgR149
PubMed Id
First Author
Ivanovska, N
Title
Bone resorption and remodeling in murine collagenase-induced osteoarthritis after administration of glucosamine.
Citation
Arthritis research & therapy; 13(2) pgR44
PubMed Id
First Author
Baud'huin, M
Title
Factor VIII-von Willebrand factor complex inhibits osteoclastogenesis and controls cell survival.
Citation
The Journal of Biological Chemistry; 284(46) pg31704-13
PubMed Id