Anti-Human sTRAIL/Apo2L 0 ReviewsSubmit a Review Product Details Catalogue Number: 500-P135 Source: Polyclonal Rabbit Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human sTRAIL/Apo2L. Anti-Human sTRAIL/Apo2L-specific antibody was purified by affinity chromatography employing immobilized Human sTRAIL/Apo2L matrix. Immunogen: E.coli derived Recombinant Human sTRAIL/Apo2L (PeproTech catalog# 310-04) Sandwich ELISA: To detect hsTRAIL/Apo2L by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech’s Biotinylated Anti-Human sTRAIL/Apo2L (500-P135Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hsTRAIL/Apo2L. Western Blot: To detect hsTRAIL/Apo2L by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hsTRAIL/Apo2L is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. Note: Additional applications tested on a lot-to-lot basis. Please contact Technical Support for more information. crossreactivity: Human, Rat References SDS CoA Search Product Line Country Of Origin: USA Not for human use. Research Interest Apoptosis Cancer Immune System TNF Superfamily product.subtitle.recentcitations First Author Tatzel, K Title Membrane-proximal TRAIL species are incapable of inducing short circuit apoptosis signaling: Implications for drug development and basic cytokine biology. Citation Scientific Reports; 6 pg22661 PubMed Id 26935795 First Author Kemp, T J Title Neutrophil stimulation with Mycobacterium bovis bacillus Calmette-Guerin (BCG) results in the release of functional soluble TRAIL/Apo-2L. Citation Blood; 106(10) pg3474-82 PubMed Id 16037389 First Author Ludwig, A T Title Tumor necrosis factor-related apoptosis-inducing ligand: a novel mechanism for Bacillus Calmette-Guérin-induced antitumor activity. Citation Cancer Research; 64(10) pg3386-90 PubMed Id 15150089
