Produced from sera of rabbits immunized with highly
pure recombinant Human TFF-2. Anti-Human
TFF-2 specific antibody was purified by affinity chromatography employing an immobilized
Human TFF-2 matrix.
E.coli derived Recombinant Human TFF-2 (PeproTech catalog# 300-59)
To detect Human TFF-2 by sandwich ELISA (using
100μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this
antibody is required. This antigen affinity purified antibody, in conjunction
with PeproTech’s Biotinylated Anti-Human TFF-2 (500-P312Bt) as a detection
antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human
To detect Human TFF-2 by Western Blot analysis
this antibody can be used at a concentration of 0.1 - 0.2 μg/ml. When used in conjunction with compatible
secondary reagents the detection limit for recombinant Human TFF-2 is 1.5 - 3.0
ng/lane, under either reducing or non-reducing conditions.
Additional applications tested on a lot-to-lot basis. Please contact Technical Support for more information.