Monoclonal Antibodies

Anti-Human TNF-α (Monoclonal Mouse)

Product Details

Catalogue Number: 500-M26

Source: Monoclonal Mouse

Preparation: Produced in BALB/c mice immunized with highly pure Recombinant Human TNF-α. Anti-Human TNF- α-specific antibody was purified from cell culture by Protein G affinity chromatography.

Immunogen: E.coli derived Recombinant Human TNF-α (PeproTech catalog# 300-01A)

Sandwich ELISA: In a sandwich ELISA (assuming 100μl/well), a concentration of 2.0-4.0 μg/ml of this antibody will detect at least 1000 pg/ml of Recombinant Human TNF-α when used with PeproTech’s Biotinylated Antigen Affinity Purified Anti-Human TNF-α (500-P31ABT) as the detection antibody at a concentration of at least 0.5-1.0 μg/ml.

Anti-Human TNF-α (Monoclonal Mouse) Sandwich ELISA

Western Blot: To detect Human TNF-α by Western Blot analysis this antibody can be used at a concentration of 0.5-1.0 μg/ml. When used in conjunction with compatible secondary reagents the detection limit for Recombinant Human TNF-α is 0.5-1.0 ng/lane, under reducing conditions and 0.25-0.5 ng/lane, under non-reducing conditions.

Anti-Human TNF-α (Monoclonal Mouse) Western Blot Reduced Anti-Human TNF-α (Monoclonal Mouse) Western Blot Unreduced

Note:

Additional applications tested on a lot-to-lot basis. Please contact Technical Support for more information.

crossreactivity:
Country Of Origin: USA

Not for human use.

Research Interest

product.subtitle.recentcitations

First Author
Oliveira, J A
Title
Mycobacterium leprae induces a tolerogenic profile in monocyte-derived dendritic cells via TLR2 induction of IDO.
Citation
Journal of Leukocyte Biology; 110(1) pg167-176
PubMed Id
First Author
Soria, G
Title
Inflammatory mediators in breast cancer: coordinated expression of TNFα & IL-1β with CCL2 & CCL5 and effects on epithelial-to-mesenchymal transition.
Citation
BMC Cancer; 11 pg130
PubMed Id
First Author
Miller, J L
Title
The type I NADH dehydrogenase of Mycobacterium tuberculosis counters phagosomal NOX2 activity to inhibit TNF-alpha-mediated host cell apoptosis.
Citation
PLoS Pathogens; 6(4) pge1000864
PubMed Id