Antigen Affinity Purified Polyclonal Antibodies

Anti-Human TNF-α (Polyclonal Goat)

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Product Details

Catalogue Number: 500-P31AG

Source: Polyclonal Goat

Preparation: Produced from sera of goats pre-immunized with highly pure recombinant Human TNF-α. Anti-Human TNF-α specific antibody was purified by affinity chromatography employing immobilized Human TNF-α matrix.

Immunogen: E.coli derived Recombinant Human TNF-α (PeproTech catalog# 300-01A)

Sandwich ELISA: To detect Human TNF-α by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech’s Biotinylated Anti-Human TNF-α (500-P31AGBt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human TNF-α.

Anti-Human TNF-α Sandwich ELISA Anti-Human TNF-α Sandwich ELISA

Western Blot: To detect Human TNF-α by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human TNF-α is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Anti-Human TNF-α Western Blot Reduced Anti-Human TNF-α Western Blot Unreduced Anti-Human TNF-α Western Blot Reduced Anti-Human TNF-α Western Blot Unreduced

Note:

Additional applications tested on a lot-to-lot basis. Please contact Technical Support for more information.

crossreactivity:
Country Of Origin: USA

Not for human use.

Research Interest

product.subtitle.recentcitations

First Author
Bugueno, I M
Title
Porphyromonas gingivalis triggers the shedding of inflammatory endothelial microvesicles that act as autocrine effectors of endothelial dysfunction.
Citation
Scientific Reports; 10(1) pg1778
PubMed Id
First Author
Kanzaki, H
Title
Soluble RANKL Cleaved from Activated Lymphocytes by TNF-α-Converting Enzyme Contributes to Osteoclastogenesis in Periodontitis.
Citation
Journal of immunology (Baltimore, Md. : 1950); 197(10) pg3871-3883
PubMed Id
First Author
Hasson, E
Title
Skin-derived micro-organs induce angiogenesis in rabbits.
Citation
Journal of Vascular Research; 43(2) pg139-48
PubMed Id