Antigen Affinity Purified Polyclonal Antibodies

Source: Polyclonal Rabbit

Preparation: Produced from sera of rabbits immunized with highly pure recombinant Human VCAM-1. Anti-Human VCAM-1 specific antibody was purified by affinity chromatography employing an immobilized Human VCAM-1 matrix.

Immunogen: HEK293 cells derived Recombinant Human VCAM-1 (PeproTech catalog# 150-04)

Sandwich ELISA: To detect hVCAM-1 by sandwich ELISA (using 100μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech’s Biotinylated Anti-Human VCAM-1 (500-P300Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hVCAM-1.

Western Blot: To detect hVCAM-1 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hVCAM-1 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Note:

Additional applications tested on a lot-to-lot basis. Please contact Technical Support for more information.