Antigen Affinity Purified Polyclonal Antibodies

Anti-Murine Eotaxin (CCL11)

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Product Details

Catalogue Number: 500-P67
Description:
Anti-Murine Eotaxin (CCL11)
 

Source: Polyclonal Rabbit

Preparation: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant mEotaxin. Anti-Murine Eotaxin specific antibody was purified by affinity chromatography employing immobilized mEotaxin matrix.

Immunogen: E.coli derived Recombinant Murine Eotaxin (CCL11) (PeproTech catalog# 250-01)

Sandwich ELISA: To detect mEotaxin by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech’s Biotinylated Anti-Murine Eotaxin (500-P67Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mEotaxin.

Anti-Murine Eotaxin (CCL11) Sandwich ELISA

Western Blot: To detect mEotaxin by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mEotaxin is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Anti-Murine Eotaxin (CCL11) Western Blot Reduced Anti-Murine Eotaxin (CCL11) Western Blot Unreduced

Note:

Additional applications tested on a lot-to-lot basis. Please contact Technical Support for more information.

crossreactivity:
Country Of Origin: USA

Not for human use.

Research Interest

product.subtitle.recentcitations

First Author
Chao, G Y
Title
Iddm30 controls pancreatic expression of Ccl11 (Eotaxin) and the Th1/Th2 balance within the insulitic lesions.
Citation
Journal of immunology (Baltimore, Md. : 1950); 192(8) pg3645-53
PubMed Id
First Author
Rose, C E
Title
Murine lung eosinophil activation and chemokine production in allergic airway inflammation.
Citation
Cellular & molecular immunology; 7(5) pg361-74
PubMed Id
First Author
Fryer, A D
Title
Neuronal eotaxin and the effects of CCR3 antagonist on airway hyperreactivity and M2 receptor dysfunction.
Citation
The Journal of Clinical Investigation; 116(1) pg228-36
PubMed Id