Antigen Affinity Purified Polyclonal Antibodies

Source: Polyclonal Rabbit

Preparation: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant mIL-21 (Murine Interleukin-21). Anti-Murine IL-21 specific antibody was purified by affinity chromatography employing immobilized mIL-21 matrix.

Immunogen: E.coli derived Recombinant Murine IL-21 (PeproTech catalog# 210-21)

Sandwich ELISA: To detect mIL-21 by sandwich ELISA (using 100μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech’s Biotinylated Anti-Murine IL-21 (500-P278Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mIL-21.

Western Blot: To detect mIL-21 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-21 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Note:

Additional applications tested on a lot-to-lot basis. Please contact Technical Support for more information.