Antigen Affinity Purified Polyclonal Antibodies

Anti-Murine IL-22

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Product Details

Catalogue Number: 500-P223

Source: Polyclonal Rabbit

Preparation: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant mIL-22. Anti-Murine IL-22 specific antibody was purified by affinity chromatography employing immobilized mIL-22 matrix.

Immunogen: E.coli derived Recombinant Murine IL-22 (PeproTech catalog# 210-22)

Sandwich ELISA: To detect mIL-22 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech’s Biotinylated Anti-Murine IL-22 (500-P223Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mIL-22.

Anti-Murine IL-22 Sandwich ELISA

Western Blot: To detect mIL-22 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-22 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Anti-Murine IL-22 Western Blot Reduced Anti-Murine IL-22 Western Blot Unreduced

Note:

Additional applications tested on a lot-to-lot basis. Please contact Technical Support for more information.

crossreactivity:
Country Of Origin: USA

Not for human use.

Research Interest

product.subtitle.recentcitations

First Author
Klooster, J P
Title
Enterocytes, fibroblasts and myeloid cells synergize in anti-bacterial and anti-viral pathways with IL22 as the central cytokine
Citation
Communications Biology; 4(1) pg631
PubMed Id
First Author
Doisne, J
Title
Cutting edge: crucial role of IL-1 and IL-23 in the innate IL-17 response of peripheral lymph node NK1.1- invariant NKT cells to bacteria.
Citation
Journal of immunology (Baltimore, Md. : 1950); 186(2) pg662-6
PubMed Id
First Author
Graham, A C
Title
IL-22 production is regulated by IL-23 during Listeria monocytogenes infection but is not required for bacterial clearance or tissue protection.
Citation
PLoS ONE; 6(2) pge17171
PubMed Id