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Antigen Affinity Purified Polyclonal Antibodies

Anti-Murine IL-3

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Product Details

Catalogue Number: 500-P53
Anti-Murine IL-3

Source: Polyclonal Rabbit

Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Murine IL-3. Anti-Murine IL-3-specific antibody was purified by affinity chromatography employing immobilized Murine IL-3 matrix.

Immunogen: E.coli derived Recombinant Murine IL-3 (PeproTech catalog# 213-13)

Sandwich ELISA: To detect mIL-3 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech’s Biotinylated Anti-Murine IL-3 (500-P53Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mIL-3.

Anti-Murine IL-3 Sandwich ELISA

Western Blot: To detect mIL-3 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-3 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Anti-Murine IL-3 Western Blot Reduced Anti-Murine IL-3 Western Blot Unreduced


Additional applications tested on a lot-to-lot basis. Please contact Technical Support for more information.

Country Of Origin: USA

Not for human use.

Research Interest


First Author
Metcalf, D
Inactivation of PU.1 in adult mice leads to the development of myeloid leukemia.
Proceedings of the National Academy of Sciences of the United States of America; 103(5) pg1486-91
PubMed Id