Antigen Affinity Purified Polyclonal Antibodies

Anti-Murine IL-4

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Product Details

Catalogue Number: 500-P54

Source: Polyclonal Rabbit

Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Murine IL-4. Anti-Murine IL-4-specific antibody was purified by affinity chromatography employing immobilized Murine IL-4 matrix.

Immunogen: E.coli derived Recombinant Murine IL-4 (PeproTech catalog# 214-14)

Sandwich ELISA: To detect Murine IL-4 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech’s Biotinylated Anti-Murine IL-4 (500-P54Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Murine IL-4.

Anti-Murine IL-4 Sandwich ELISA

Western Blot: To detect Murine IL-4 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Murine IL-4 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Anti-Murine IL-4 Western Blot Reduced Anti-Murine IL-4 Western Blot Unreduced


Additional applications tested on a lot-to-lot basis. Please contact Technical Support for more information.

Country Of Origin: USA

Not for human use.

Research Interest


First Author
Polesso, F
Constitutive expression of NF-κB inducing kinase in regulatory T cells impairs suppressive function and promotes instability and pro-inflammatory cytokine production.
Scientific Reports; 7(1) pg14779
PubMed Id
First Author
Okoye, I S
Transcriptomics identified a critical role for Th2 cell-intrinsic miR-155 in mediating allergy and antihelminth immunity.
Proceedings of the National Academy of Sciences of the United States of America; 111(30) pgE3081-90
PubMed Id
First Author
Maldonado Galdeano, C
Impact of a probiotic fermented milk in the gut ecosystem and in the systemic immunity using a non-severe protein-energy-malnutrition model in mice.
BMC Gastroenterology; 11 pg64
PubMed Id