Antigen Affinity Purified Polyclonal Antibodies

Anti-Murine IL-6

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Product Details

Catalogue Number: 500-P56
Description:
Anti-Murine IL-6
 

Source: Polyclonal Rabbit

Preparation: Produced from sera of rabbits pre-immunized with highly pure recombinant Murine IL-6. Anti-Murine IL-6 specific antibody was purified by affinity chromatography employing immobilized Murine IL-6 matrix.

Immunogen: E.coli derived Recombinant Murine IL-6 (PeproTech catalog# 216-16)

Sandwich ELISA: To detect Murine IL-6 by sandwich ELISA (using 100μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech’s Biotinylated Anti-Murine IL-6 (500-P56Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Murine IL-6.

Anti-Murine IL-6 Sandwich ELISA

Western Blot: To detect Murine IL-6 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Murine IL-6 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Anti-Murine IL-6 Western Blot Reduced Anti-Murine IL-6 Western Blot Unreduced

Note:

Additional applications tested on a lot-to-lot basis. Please contact Technical Support for more information.

crossreactivity:
Country Of Origin: USA

Not for human use.

Research Interest

product.subtitle.recentcitations

First Author
Klooster, J P
Title
Enterocytes, fibroblasts and myeloid cells synergize in anti-bacterial and anti-viral pathways with IL22 as the central cytokine
Citation
Communications Biology; 4(1) pg631
PubMed Id
First Author
Choi, S
Title
Relative Contributions of B Cells and Dendritic Cells from Lupus-Prone Mice to CD4
Citation
Journal of immunology (Baltimore, Md. : 1950); 200(9) pg3087-3099
PubMed Id
First Author
Scarlett, U K
Title
Ovarian cancer progression is controlled by phenotypic changes in dendritic cells.
Citation
The Journal of Experimental Medicine; 209(3) pg495-506
PubMed Id