Anti-Murine Leptin 0 ReviewsSubmit a Review Product Details Catalogue Number: 500-P68 Description: Source: Polyclonal Rabbit Preparation: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant mLeptin. Anti-Murine Leptin specific antibody was purified by affinity chromatography employing immobilized mLeptin matrix. Immunogen: E.coli derived Recombinant Murine Leptin (PeproTech catalog# 450-31) Sandwich ELISA: To detect mLeptin by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech’s Biotinylated Anti-Murine Leptin (500-P68Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mLeptin. Western Blot: To detect mLeptin by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mLeptin is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. Note: Additional applications tested on a lot-to-lot basis. Please contact Technical Support for more information. crossreactivity: Mouse, References SDS CoA Search Product Line Country Of Origin: USA Not for human use. Research Interest Angiogenesis/Cardiovascular Apoptosis Diabetes/Weight Regulation Inflammation Wound Healing product.subtitle.recentcitations First Author Harrison, L Title Fluorescent blood-brain barrier tracing shows intact leptin transport in obese mice. Citation International journal of obesity (2005); 43(6) pg1305-1318 PubMed Id 30283080 First Author Ronkainen, J Title Fat mass- and obesity-associated gene Fto affects the dietary response in mouse white adipose tissue. Citation Scientific Reports; 5 pg9233 PubMed Id 25782772
