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Antigen Affinity Purified Polyclonal Antibodies

Anti-Murine MCP-3 (CCL7)

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Product Details

Catalog Number: 500-P116G
Description:
Anti-Murine MCP-3 (CCL7)
 

Source: Polyclonal Goat

Preparation: Produced from sera of goats pre-immunized with highly pure (>98%) recombinant mMCP-3. Anti-Murine MCP-3 specific antibody was purified by affinity chromatography employing immobilized mMCP-3 matrix.

Immunogen: E.coli derived Recombinant Murine MCP-3 (CCL7) (PeproTech catalog# 250-08)

Sandwich ELISA: To detect mMCP-3 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech’s Biotinylated Anti-Murine MCP-3 (500-P116GBt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mMCP-3.

Anti-Murine MCP-3 (CCL7) Sandwich ELISA

Western Blot: To detect mMCP-3 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mMCP-3 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Anti-Murine MCP-3 (CCL7) Western Blot Reduced Anti-Murine MCP-3 (CCL7) Western Blot Unreduced

Note:

Additional applications tested on a lot-to-lot basis. Please contact Technical Support for more information.

Cross Reactivity Cited in References:
Country Of Origin: USA

Not for human use.

Research Interest

Recent Citations

First Author
José, R J
Title
Regulation of neutrophilic inflammation by proteinase-activated receptor 1 during bacterial pulmonary infection.
Citation
Journal of immunology (Baltimore, Md. : 1950); 194(12) pg6024-34
PubMed ID
First Author
Tamba, S
Title
Timely interaction between prostaglandin and chemokine signaling is a prerequisite for successful fertilization.
Citation
Proceedings of the National Academy of Sciences of the United States of America; 105(38) pg14539-44
PubMed ID
First Author
Michalec, L
Title
CCL7 and CXCL10 orchestrate oxidative stress-induced neutrophilic lung inflammation.
Citation
Journal of immunology (Baltimore, Md. : 1950); 168(2) pg846-52
PubMed ID