Anti-Murine VEGF165 0 ReviewsSubmit a Review Product Details Catalog Number: 500-P131 Description: Source: Polyclonal Rabbit Preparation: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant mVEGF. Anti-Murine VEGF specific antibody was purified by affinity chromatography employing immobilized mVEGF matrix. Immunogen: E.coli derived Recombinant Murine VEGF165 (PeproTech catalog# 450-32) Sandwich ELISA: To detect mVEGF by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech’s Biotinylated Anti-Murine VEGF (500-P131Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mVEGF. Western Blot: To detect mVEGF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mVEGF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. Note: Additional applications tested on a lot-to-lot basis. Please contact Technical Support for more information. Cross Reactivity Cited in References: Mouse References SDS CoA Search Product Line Country Of Origin: USA Not for human use. Research Interest Angiogenesis/Cardiovascular Cancer Inflammation Proliferation Recent Citations First Author Bussard, K M Title Osteoblasts are a major source of inflammatory cytokines in the tumor microenvironment of bone metastatic breast cancer. Citation Journal of Cellular Biochemistry; 111(5) pg1138-48 PubMed ID 20683902 First Author Hasson, E Title A cell-based multifactorial approach to angiogenesis. Citation Journal of Vascular Research; 42(1) pg29-37 PubMed ID 15627784
