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Antigen Affinity Purified Polyclonal Antibodies

Anti-Murine VEGF165

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Product Details

Catalogue Number: 500-P131
Anti-Murine VEGF 165

Source: Polyclonal Rabbit

Preparation: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant mVEGF. Anti-Murine VEGF specific antibody was purified by affinity chromatography employing immobilized mVEGF matrix.

Immunogen: E.coli derived Recombinant Murine VEGF165 (PeproTech catalog# 450-32)

Sandwich ELISA: To detect mVEGF by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech’s Biotinylated Anti-Murine VEGF (500-P131Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mVEGF.

Anti-Murine VEGF 165 Sandwich ELISA

Western Blot: To detect mVEGF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mVEGF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Anti-Murine VEGF 165 Western Blot Reduced Anti-Murine VEGF 165 Western Blot Unreduced


Additional applications tested on a lot-to-lot basis. Please contact Technical Support for more information.

Country Of Origin: USA

Not for human use.

Research Interest


First Author
Bussard, K M
Osteoblasts are a major source of inflammatory cytokines in the tumor microenvironment of bone metastatic breast cancer.
Journal of Cellular Biochemistry; 111(5) pg1138-48
PubMed Id
First Author
Hasson, E
A cell-based multifactorial approach to angiogenesis.
Journal of Vascular Research; 42(1) pg29-37
PubMed Id