Antigen Affinity Purified Polyclonal Antibodies

Anti-Rat IFN-γ (Polyclonal Goat)

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Product Details

Catalogue Number: 500-P122G
Description:
Anti-Rat IFN-γ (Polyclonal Goat)
 

Source: Polyclonal Goat

Preparation: Produced from sera of goats pre-immunized with highly pure recombinant Rat IFN-γ. Anti-Rat IFN-γ specific antibody was purified by affinity chromatography employing immobilized Rat IFN-γ matrix.

Immunogen: E.coli derived Recombinant Rat IFN-γ (PeproTech catalog# 400-20)

Sandwich ELISA: To detect Rat IFN-γ by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech’s Biotinylated Anti-Rat IFN-γ (500-P122GBt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat IFN-γ.

Anti-Rat IFN-γ Sandwich ELISA

Western Blot: To detect Rat IFN-γ by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Rat IFN-γ is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Anti-Rat IFN-γ Western Blot Reduced Anti-Rat IFN-γ Western Blot Unreduced

Note:

Additional applications tested on a lot-to-lot basis. Please contact Technical Support for more information.

crossreactivity:
Rat
Country Of Origin: USA

Not for human use.

Research Interest

product.subtitle.recentcitations

First Author
Zinöcker, S
Title
Rat mesenchymal stromal cells inhibit T cell proliferation but not cytokine production through inducible nitric oxide synthase.
Citation
Frontiers in Immunology; 3 pg62
PubMed Id