Antigen Affinity Purified Polyclonal Antibodies

Anti-Rat IL-6 (Polyclonal Rabbit)

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Product Details

Catalogue Number: 500-P73

Source: Polyclonal Rabbit

Preparation: Produced from sera of rabbits pre-immunized with highly pure recombinant Rat IL-6. Anti-Rat IL-6 specific antibody was purified by affinity chromatography employing immobilized Rat IL-6 matrix.

Immunogen: E.coli derived Recombinant Rat IL-6 (PeproTech catalog# 400-06)

Sandwich ELISA: To detect Rat IL-6 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech’s Biotinylated Anti-Rat IL-6 (500-P73Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat IL-6.

Anti-Rat IL-6 Sandwich ELISA Anti-Rat IL-6 Sandwich ELISA

Western Blot: To detect Rat IL-6 by Western Blot analysis this antibody can be used at a concentration of 0.1- 0.2 µg/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Rat IL-6 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Anti-Rat IL-6 Western Blot Reduced Anti-Rat IL-6 Western Blot Unreduced Anti-Rat IL-6 Western Blot Reduced Anti-Rat IL-6 Western Blot Unreduced

Note:

Additional applications tested on a lot-to-lot basis. Please contact Technical Support for more information.

crossreactivity:
Rat
Country Of Origin: USA

Not for human use.

Research Interest

product.subtitle.recentcitations

First Author
Chagnon, F
Title
Endotoxin-induced myocardial dysfunction: effects of macrophage migration inhibitory factor neutralization.
Citation
Circulation Research; 96(10) pg1095-102
PubMed Id
First Author
Liu, Z
Title
A novel role for nuclear factor of activated T cells in receptor tyrosine kinase and G protein-coupled receptor agonist-induced vascular smooth muscle cell motility.
Citation
The Journal of Biological Chemistry; 279(39) pg41218-26
PubMed Id
First Author
Zinöcker, S
Title
Mycoplasma contamination revisited: mesenchymal stromal cells harboring Mycoplasma hyorhinis potently inhibit lymphocyte proliferation in vitro.
Citation
PLoS ONE; 6(1) pge16005
PubMed Id