Biotinylated Anti-Human BD-3 0 ReviewsSubmit a Review Product Details Catalog Number: 500-P241BT Description: Source: Polyclonal Rabbit Preparation: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hBD-3. Anti-Human BD-3 specific antibody was purified by affinity chromatography and then biotinylated. Immunogen: E.coli derived Recombinant Human BD-3 (PeproTech catalog# 300-52) Sandwich ELISA: To detect hBD-3 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech’s Polyclonal Anti-Human BD-3 (500-P241) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant hBD-3. Western Blot: To detect hBD-3 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hBD-3 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. Note: 1mg will be provided as 2x500μg. Cross Reactivity Cited in References: Human References SDS CoA Search Product Line Country Of Origin: USA Not for human use. Research Interest Chemotaxis Immune System Recent Citations First Author Jin, G Title An antimicrobial peptide regulates tumor-associated macrophage trafficking via the chemokine receptor CCR2, a model for tumorigenesis. Citation PLoS ONE; 5(6) pge10993 PubMed ID 20544025 First Author Kalus, A A Title Association of a genetic polymorphism (-44 C/G SNP) in the human DEFB1 gene with expression and inducibility of multiple beta-defensins in gingival keratinocytes. Citation BMC Oral Health; 9 pg21 PubMed ID 19712472