Produced from sera of rabbits pre-immunized with highly pure (>98%)
recombinant hBD-5. Anti-Human BD-5 specific antibody was purified by
affinity chromatography and then biotinylated.
E.coli derived Recombinant Human BD-5 (PeproTech catalog# 300-68)
To detect hBD-5 by sandwich ELISA (using 100 μl/well antibody solution) a
concentration of 0.25 – 1.0 μg/ml of this antibody is required. This
biotinylated polyclonal antibody, in conjunction with PeproTech’s
Polyclonal Anti-Human BD-5 (500-P323) as a capture antibody, allows the
detection of at least 0.2 – 0.4 ng/well of recombinant hBD-5.
To detect hBD-5 by Western Blot analysis this antibody can be used at a
concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible
secondary reagents the detection limit for recombinant hBD-5 is 1.5 - 3.0
ng/lane, under either reducing or non-reducing conditions.
1mg will be provided as 2x500μg.