Produced from sera of rabbits immunized with highly pure Recombinant Human EPO. Anti-Human EPO-specific antibody was purified by affinity chromatography and then biotinylated.
CHO cells derived Recombinant Human EPO (PeproTech catalog# 100-64)
To detect Human EPO by sandwich ELISA (using 100μl/well), a concentration of 0.25-1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech’s Polyclonal Anti-Human EPO (500-P318) as a capture antibody, allows the detection of at least 2000-4000 pg/ml of Recombinant Human EPO.
To detect Human EPO by Western Blot analysis, this antibody can be used at a concentration of 0.1-0.2 μg/ml. When used in conjunction with compatible development reagents, the detection limit for Recombinant Human EPO is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
1mg will be provided as 2x500μg.
Not for human use.