Biotinylated Anti-Human FGF-23 0 ReviewsSubmit a Review Product Details Catalog Number: 500-P319BT Source: Polyclonal Rabbit Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human FGF-23. Anti-Human FGF-23-specific antibody was purified by affinity chromatography and then biotinylated. Immunogen: E.coli-derived, 25.5kDa Recombinant Human FGF-23 (PeproTech Catalog# 100-52) Sandwich ELISA: To detect Human FGF-23 by sandwich ELISA (using 100ul/well), a concentration of 0.25-1.0 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech’s Polyclonal Anti-Human FGF-23 (500-P319) as a capture antibody, allows the detection of at least 2000-4000 pg/ml of Recombinant Human FGF-23. Western Blot: To detect Human FGF-23 by Western Blot analysis, this antibody can be used at a concentration of 0.1-0.2ug/ml. When used in conjunction with compatible development reagents, the detection limit for Recombinant Human FGF-23 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. Note: 1mg will be provided as 2x500μg. SDS CoA Search Product Line Country Of Origin: USA Not for human use. Research Interest Angiogenesis/Cardiovascular Neurobiology Stem Cells & Differentiation Bone, Skeletal, Cartilage Cancer FGF Superfamily Immune System Inflammation Wound Healing