Biotinylated Anti-Human IFN-β 0 ReviewsSubmit a Review Product Details Catalog Number: 500-P32BBT Description: Source: Polyclonal Rabbit Preparation: Produced from sera of rabbits pre-immunized with highly pure recombinant Human IFN-β. Anti-Human IFN-β specific antibody was purified by affinity chromatography and then biotinylated. Immunogen: E.coli derived Recombinant Human IFN-β (PeproTech catalog# 300-02B)* *Catalog# 300-02B has been replaced with 300-02BC. Sandwich ELISA: To detect Human IFN-β by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech’s Polyclonal Anti-Human IFN-β (500-P32B) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant Human IFN-β. Western Blot: To detect Human IFN-β by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human IFN-β is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. Note: 1mg will be provided as 2x500μg. Cross Reactivity Cited in References: Human References SDS CoA Search Product Line Country Of Origin: USA Not for human use. Research Interest COVID-19 AIDS/HIV Cancer Immune System Neurobiology Recent Citations First Author Kölle, J Title Respiratory infections regulated blood cells IFN-β-PD-L1 pathway in pediatric asthma. Citation Immunity, Inflammation and Disease; 8(3) pg310-319 PubMed ID 32394602 First Author Siednienko, J Title Nuclear factor κB subunits RelB and cRel negatively regulate Toll-like receptor 3-mediated β-interferon production via induction of transcriptional repressor protein YY1. Citation The Journal of Biological Chemistry; 286(52) pg44750-63 PubMed ID 22065573
